• By Inessa Peters
  • September 30, 2025
  • No Comments

Overlay Function

If you need to compare multiple Channels or Results side by side, Empower’s Overlay function makes it easy.

Here’s how:

1️⃣ In your Project Window, select 2 or more Channels or Results and click Review.

2️⃣ In the Review window, you’ll first see only one chromatogram.

👉 Tip: Add a Legend so you know exactly which sample you’re viewing.

3️⃣ Open the 2D Channels tab below for a clear overview – you can adjust the column order to your preference.

4️⃣ Right-click in the chromatogram → Properties → go to the Overlay tab.

Now choose how you want to view your chromatograms:

  • Do Not Overlay – shows one chromatogram at a time
  • Make Stack Plot – displays all chromatograms stacked in separate windows. Use the Visible checkbox to show/hide channels. Great for comparing samples.
  • Overlay in Single Plot – overlays all chromatograms into one window. You can still control visibility for clarity – ideal for comparing your samples with blanks.

⚙️ Chromatogram Annotation setting:

  • All Chroms in Overlay – Integration and Peak Names are visible in all chromatograms (works best with Stack Plot)
  • Only One Chrom in Overlay – Integration and Peak Names are visible in the selected chromatogram (recommended for Single Plot)

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